654205 TNF-α, Human, Recombinant, E. coli

654205
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654205-10UGCN
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      Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
      Catalogue Number654205
      Brand Family Calbiochem®
      SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
      References
      ReferencesMerck Index 13, 9882.
      Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
      Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
      Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
      Koide, M., et al. 1993. FEBS Lett. 318, 213.
      Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
      Product Information
      CAS number94948-59-1
      FormLyophilized
      FormulationCarrier-free
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSMT6435000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TNF-α, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      654205

      References

      Reference overview
      Merck Index 13, 9882.
      Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
      Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
      Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
      Koide, M., et al. 1993. FEBS Lett. 318, 213.
      Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-January-2009 JSW
      SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
      DescriptionRecombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
      FormLyophilized
      FormulationCarrier-free
      CAS number94948-59-1
      RTECSMT6435000
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
      Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
      SolubilityReconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
      Toxicity Standard Handling
      ReferencesMerck Index 13, 9882.
      Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
      Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
      Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
      Koide, M., et al. 1993. FEBS Lett. 318, 213.
      Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.