70073 pET-30 Xa/LIC Vector Kit - Novagen

70073
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      Description
      OverviewThe pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70073
      Brand Family Novagen®
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      Quality LevelMQ100
      Applications
      Biological Information
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      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      pET-30 Xa/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      70073

      Citations

      Title
    • Jian Zhang, et al. (2008) A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Procedings of the National Academy of Science 105, 1460-1465.
    • Purnima Singh, et al. (2007) Concerted action of exonuclease and gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments. Journal of Biological Chemistry 282, 3465-3477.
    • Spencer M. Whitney and Robert E. Sharwood. (2007) Linked rubisco subunits can assemble into functional oligomers without impeding catalytic performance. Journal of Biological Chemistry 282, 3809-3818.
    • Gianguido Coffa, et al. (2005) On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant. Journal of Biological Chemistry 280, 38756-38766.
    • Jason Gunawan, et al. (2005) Structural and mechanistic analysis of sialic acid synthase neuB from Neisseria meningitidis in complex with Mn2+, phosphoenolpyruvate, and n-acetylmannosaminitol. Journal of Biological Chemistry 280, 3555-3563.
    • Steven G. Van Lanen, et al. (2005) From cyclohydrolase to oxidoreductase: Discovery of nitrile reductase activity in a common fold. Proceedings of the National Academy of Sciences (USA) 102, 4264-4269.
    • Domenico Sanfelice, et al. (2004) Letter to the editor: resonance assignment and secondary structire of the La motif. Journal of Biomolecular NMR 29, 449-450.
    • Antonio Garofalo, et al. (2003) The FAR protein family of the nematode Caenorhabditis elegans. Differential lipid binding properties, structural characteristics, and developmental regulation. Journal of Biological Chemistry 278, 8065-8074.
    • Michael B. Murphy, et al. (2003) An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Research 31, e110-.
    • Jennifer S. Laurence, et al. (2000) CC Chemokine MIP-1 can function as a monomer and depends on Phe13 for receptor binding. Biochemistry 39, 3401-3409.
    • User Protocols

      Title
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Vector Map

      Title
      TB185VM pET-30 Xa/LIC Vector Map

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