70072 pET-32 Xa/LIC Vector Kit - Novagen

70072
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      Description
      OverviewThe pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70072
      Brand Family Novagen®
      References
      Product Information
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      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
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      Safety Information according to GHS
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      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      pET-32 Xa/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      70072

      Citations

      Title
    • Hongjun Jin, et al. (2007) The human CC chemokine MIP-1 dimer is not competent to bind to the CCR5 receptor. Journal of Biological Chemistry 282, 27976-27983.
    • Geoffrey A. Preidis, et al. (2007) Seropositive human subjects produce interferon gamma after stimulation with recombinant Cryptosporidium hominis gp15. American Journal of Tropical Medicine and Hygiene 77, 583-585.
    • Beatrice Alpha-Bazin, et al. (2005) Boundaries and physical characterization of a new domain shared between mammalian 53BP1 and yeast Rad9 checkpoint proteins. Protein Science 14, 1827-1839.
    • Rey-Ting Guo, et al. (2005) Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. Journal of Biological Chemistry 280, 20762-20774.
    • Min-Feng Hsu, et al. (2005) Understanding the maturation process and inhibitor design of SARS-CoV 3CLpro from the crystal structure of C145A in a product-bound form. Journal of Biological Chemistry 280, 31257-31266.
    • Savvas Saouros, et al. (2005) A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell-adhesion complex. Journal of Biological Chemistry 280, 38583-38591.
    • Atsushi Kodan, Hiroyuki Kuroda and Fukumi Sakai. (2002) A stilbene synthase from Japanese red pine (Pinus densiflora): Implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis. Procedings of the National Academy of Science 99, 3335-3339.
    • User Protocols

      Title
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Vector Map

      Title
      TB186VM pET-32 Xa/LIC Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors