70840 pTriEx™-1.1 DNA - Novagen

70840
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70840-3CN
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      Plastic ampoule 20 μg
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      Description
      OverviewThe pTriEx™-1.1 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-1.1 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λ>pI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction.







      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70840
      Brand Family Novagen®
      References
      Product Information
      DeclarationThis product is not available in Japan.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pTriEx™-1.1 DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pTriEx™-1.1 DNA - Novagen Certificates of Analysis

      TitleLot Number
      70840

      Brochure

      Title
      Insect Cell Expression

      Citations

      Title
    • Cao, Y., et al. 2008. Molecular Cancer Research 6, 119.
    • Grillo, C., et al. 2007. Journal of Biological Chemistry 282, 10299.
    • Popescu, C.I., et al. 2006. Journal of Biological Chemistry 281, 21682.
    • Cao, Y., et al. 2005. Cancer Research 65, 1124.
    • Popescu, C.I., et al. 2005. Journal of Biological Chemistry 280, 13833.
    • Branza-Nichita, N., et al. 2004. Biochemical and Biophysical Research Communications 319, 655.
    • Wurm, T., et al. 2001. Journal of Virology 75, 9345.
    • User Protocols

      Title
      TB250 pTriEx™ System Manual

      Vector Map

      Title
      TB280 pTriEx™-1.1 Vector Map

      Vector Sequence

      Title
      pTri-Ex-1.1 Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors