70824 pTriEx™-4 DNA - Novagen

70824
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      Plastic ampoule 20 μg
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      Description
      OverviewThe pTriEx™-4 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by the CMV immediate early enhancer and promoter2. For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λpL and λlpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site, or native protein can be generated by cloning into the PshAI or SmaI sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit.


      2 The CMV promoter is covered by U.S. Patent nos. 5,168,062 and 5,385,839 issued to the University of Iowa Research Foundation and is licensed for research use only.





      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70824
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications

      Documentation

      pTriEx™-4 DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pTriEx™-4 DNA - Novagen Certificates of Analysis

      TitleLot Number
      70824

      Citations

      Title
    • Masumi Eto, et al. (2007) Association of the tensin N-terminal protein-tyrosine phosphatase domain with alpha isoform of protein phosphatase-1 in focal adhesions. Journal of Biological Chemistry 282, 17806-17815.
    • Masahiro Nakajima, et al. (2007) Mechanisms for asporin function and regulation in articular cartilage. Journal of Biological Chemistry 282, 32185-32192.
    • Lauren E. Ball, Mary N. Berkaw and Maria G. Buse. (2005) Identification of the major site of O-linked ß-N-acetylglucosamine modification in the C-terminus of insulin receptor substrate-1. Molecular and Cellular Proteomics 5, 313-323.
    • Paul Frankel, et al. (2005) RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity. European Molecular Biology Organization Journal 24, 54-62.
    • Kazuki Okamoto and Fumihide Isohashi. (2005) Macromolecular-translocation inhibitor-II (Zn2+-binding protein, parathymosin) interacts with the glucocorticoid receptor and enhances transcription in vivo. Journal of Biological Chemistry 280, 36986-36993.
    • Keizo Takao, et al. (2005) Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons. Journal of Neuroscience 25, 3107-3112.
    • Michael R Taylor, et al. (2005) The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells. Genetics 170, 263-273.
    • James P. Vaughn, et al. (2005) The DEXH protein product of the DHX36 gene is the major source of tetramolecular quadruplex G4-DNA resolving activity in HeLa cell lysates. Journal of Biological Chemistry 280, 38117-38120.
    • Naoya Suematsu, Kazuki Okamoto and Fumihide Isohashi. (2002) Mouse cytosolic acetyl-CoA hydrolase, a novel candidate for a key enzyme involved in fat metabolism: cDNA cloning, sequencing and functional expression. Acta Biochimica Polonica 49, 937-945.
    • User Protocols

      Title
      TB250 pTriEx™ System Manual

      Vector Map

      Title
      TB282 pTriEx™-4 Vector Map

      Vector Sequence

      Title
      pTriEx™-4 Ek/LIC Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors