Chromolith® SemiPrep HPLC Columns
Chromolith® SemiPrep HPLC columns are ideally suited for direct scale-up from analytical to semi-prep. They offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles. The columns are also excellent for solving separation problems with non-polar basic and acidic compounds, as well as peptides.
With an internal diameter (i.d.) of 10 mm, Chromolith® SemiPrep columns are the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d). Furthermore, the selectivity of Chromolith® RP-18 endcapped SemiPrep columns is comparable to common RP-18 endcapped reversed phase columns.
The columns also offer an extended lifetime thanks to their rugged monolithic silica structure, which is the same as that of Chromolith® analytical columns with an internal diameter of 4.6 mm. The 2 μm macropores of the silica rod dramatically reduce separation time, while the 13 nm mesopores create a large surface area for adsorption of target molecules.
Chromolith® SemiPrep RP-18 endcapped 100-10 mm i.d. columns combine high separation speed with excellent performance. They are the ideal alternative to particulate columns with 10 and 21.2 mm i.d.
Separation of a standard mixture
Chromolith® SemiPrep HPLC columns deliver sharp separations, even at high sample loading.
A Chromolith® SemiPrep RP-18 endcapped column injected with a 25 mg sample demonstrates the same excellent separation properties as a corresponding analytical column.
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