Skip to Content
Merck

Key Documents

View All Documentation

1.10338

Fractogel® EMD Chelate (M)

for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

Synonym(s):

Fractogel® EMD Chelate (M)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
41115711
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

ligand

iminodiacetic acid

Quality Level

400

form

resin

purified by

immobilized metal affinity chromatography (IMAC)

manufacturer/tradename

Fractogel®

parameter

8 bar max. pressure, ~240 cm/hr flow rate

matrix active group

methacrylate

wide

40-90 μm

capacity

~60 mg binding capacity( lysozyme/ml of resin)

transition temp

flash point 35 °C (calculated)

application(s)

recombinant protein

separation technique

affinity

storage temp.

2-30°C

General description

Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

Features and Benefits

Fractogel® EMD Chelate (M) enables users to:

  • Efficiently purify histidine-tagged recombinant proteins
  • Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
  • Ensure timely and successful startup and operation
  • Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
  • Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

Packaging

  • 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml
  • 1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml
  • 1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml
  • 1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L

Plastic bottle

Analysis Note

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under Fractogel® EMD Chelate (M)

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

95.0 °F

flash_point_c

35 °C

Stay compliant with GMP using the Emprove® Program

Accelerate qualification, maintain compliance, and save time with Emprove® Dossiers.

Download Dossiers

Note: you will be brought to EmproveSuite.com. Your username and password are separate from SigmaAldrich.com and may differ.

Register for free and access Emprove® Dossiers within minutes. Upgrade your subscription plan to unlock advanced and customized content in the Emprove® Suite

Not finding what you are looking for? Visit the Emprove® Program Help page.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library