C7594
Casein Blocking Buffer
for Northern and Southern blotting, powder blend
Synonym(s):
blocking buffer
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About This Item
UNSPSC Code:
12161700
EC Number:
232-555-1
NACRES:
NA.25
grade
Molecular Biology
Quality Level
form
powder blend
technique(s)
Southern blotting: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
storage temp.
room temp
General description
Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.
Application
Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.
Features and Benefits
- Better blocking performance than dried milk or BSA
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
Preparation Note
Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.
wgk
WGK 1
Storage Class
11 - Combustible Solids
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Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
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