A3678

Ammonium persulfate

Name: Ammonium persulfate
Brand: SIGMA

Molecular Biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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All Photos(3)

About This Item

Linear Formula:

(NH₄)₂S₂O₈

CAS Number:

7727-54-0

Molecular Weight:

228.20

PubChem Substance ID:

24890800

eCl@ss:

38030421

UNSPSC Code:

41105319

NACRES:

NB.22

EC Number:

231-786-5

MDL number:

MFCD00003390

Product Name

Ammonium persulfate, Molecular Biology, suitable for electrophoresis, ≥98%

assay

≥98%

form

powder

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

grade

Molecular Biology

vapor density

7.9 (vs air)

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl^-): <10 ppm

cation traces

Fe: <10 ppm
heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected
Protease, none detected
RNAse, none detected

Quality Level

200

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Related Categories

Chemical Synthesis

Oxidation Reagents

Analysis Note

Tested for use in acrylamide polymerization.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

pictograms

GHS03,GHS08,GHS07

signalword

Danger

hcodes

H272,H302,H315,H317,H319,H334,H335

pcodes

P210 - P220 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number

0000518373

0000511748

0000490889

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Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.

Chiang MY et al.

Biomaterials, 34, 9754-9754 (2013)

Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.

Fancy DA and Kodadek T

Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)

Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.

Liao IC et al.

Advances in Functional Materials, 23, 5833-5833 (2013)

Maurer HR

Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)

Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.

Alvarez-Gonzalez CA, et al.

Fish Physiology and Biochemistry, 36, 29-29 (2010)

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