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About This Item
NACRES:
NA.31
UNSPSC Code:
41105300
Storage temp.:
2-8°C
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN3005
storage temp.
2-8°C
General description
ECL™ Direct Labeling Module.
Application
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ECL is a trademark of Cytiva
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Storage Class
12 - Non Combustible Liquids
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Articles
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
Kimiko Yamamoto et al.
Genome biology, 9(1), R21-R21 (2008-01-30)
In 2004, draft sequences of the model lepidopteran Bombyx mori were reported using whole-genome shotgun sequencing. Because of relatively shallow genome coverage, the silkworm genome remains fragmented, hampering annotation and comparative genome studies. For a more complete genome analysis, we
Imane El Meouche et al.
PloS one, 8(12), e83748-e83748 (2013-12-21)
Clostridium difficile intestinal disease is mediated largely by the actions of toxins A (TcdA) and B (TcdB), whose production occurs after the initial steps of colonization involving different surface or flagellar proteins. In B. subtilis, the sigma factor SigD controls
Akiko Uemori et al.
Anticancer research, 28(1A), 91-95 (2008-04-04)
The non-insecticidal Bacillus thuringiensis strain B0195 produces parasporin (PS) whose cytocidal activity is preferential for human cancer cells. This study identified two ps genes, ps1Aa3 and ps1Ab1, from the strain B0195. The former gene was 2,169-bp long, encoding an 81