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Merck

E3004

Extract-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

Synonym(s):

Plant direct PCR master mix, Tissue direct PCR master mix

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About This Item

NACRES:
NA.55
UNSPSC Code:
41106303
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Quality Level

usage

sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications

feature

dNTPs included, hotstart, Direct PCR

technique(s)

PCR: suitable

color

colorless

input

crude DNA

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

Extract-N-Amp PCR ReadyMix is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.

Application

Extract-N-Amp PCR ReadyMix has been used for the following applications:
  • Genomic DNA extraction
  • Fungal DNA extraction and amplification by polymerase chain reaction (PCR) reactions
  • Direct PCR amplification
  • Genotyping
  • Real-Time PCR

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC


Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Extract-N-Amp™ Tissue PCR Kit quickly extracts PCR-ready DNA from mouse tails, providing optimized PCR mix for efficient amplification.


Xuan Song et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(32), 6378-6394 (2019-06-14)
ATM (ataxia-telangiectasia mutated) is a PI3K-like kinase best known for its role in the DNA damage response (DDR), especially after double-strand breaks. Mutations in the ATM gene result in a condition known as ataxia-telangiectasia (A-T) that is characterized by cancer
Muhammad Iqbal et al.
Genome, 50(5), 511-516 (2007-07-07)
Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with
Anneke van der Zee et al.
PloS one, 11(3), e0150755-e0150755 (2016-03-10)
The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S