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Merck

XTGHP-RO

Roche

X-tremeGENE™ HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

Synonym(s):

transfection reagent

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About This Item

NACRES:
NA.55
UNSPSC Code:
41106502

Key Documents

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grade

Molecular Biology

form

liquid (aqueous solution)

usage

1 mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06366244001), pkg of 1.0 mL (06366236001), pkg of 5 × 1 mL (06366546001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

Quality Level

100

Related Categories

Analysis Note

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

  • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
  • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
  • Generate physiologically relevant data using a reagent with low cytotoxic effects.
  • Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Legal Information

X-tremeGENE is a trademark of Roche

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

334.4 °F

flash_point_c

168 °C

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Certificates of Analysis (COA)

Lot/Batch Number
43940400
42980400
40344500
28049700
11876400

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Takashi Yoshino et al.
Cellular and molecular neurobiology, 31(7), 1009-1020 (2011-04-27)
A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
Abazar Roustazadeh et al.
SpringerPlus, 5(1), 711-711 (2016-07-05)
Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799
Laura M Griffin et al.
Methods in molecular biology (Clifton, N.J.), 1195, 219-238 (2013-11-28)
Human papillomaviruses (HPVs) are non-enveloped DNA viruses that are highly tropic for mucosal and cutaneous epithelia. The HPV life cycle is tightly linked to epithelial cell differentiation, where HPVs only infect the basal proliferating keratinocytes, and progeny virus assembly and
Ido D Weiss et al.
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
Jihoon Kim et al.
Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
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Articles

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

細胞へのトランスフェクション入門

トランスフェクションは、遺伝子を細胞に導入して、遺伝子発現や細胞生物学の研究に役立ちます。

トランスフェクションのしくみ

このウェビナーでは、トランスフェクションの概要および真核細胞にDNAやRNAを導入する方法について説明します。

タンパク質発現系

遺伝子工学により、研究のための遺伝子組換えタンパク質の大規模な発現・単離が可能になります。

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Protocols

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

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