Anti-Inversin Antibody

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Inversin in 10 µg of HEK293 cell lysate.

Anti-Inversin Antibody detects level of Inversin & has been published & validated for use in Western Blotting & IHC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blot Analysis: 1 µg/mL from a representative lot detected Inversin in 10 µg of human liver tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Inversin in human kidney tissue.

No homology to Rat was found.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Inversin is a tubulin-associated protein, which is highly expressed in the cilia of renal tubules. It contains sixteen ankyrin repeats at the N-terminus and two IQ domains at the C-terminus. The ankyrin repeats facilitate protein-protein interactions, whereas the IQ domains are involved in calmodulin binding. Previous studies have indicated that inversin modulates the Wnt signaling pathway, and interacts with a plethora of intracellular signaling targets, including Disheveled, Frizzled-8, alpha- and beta catenins, NPHP1, and NPHP3. Inversin is localized to mitotic spindle fibers, and plays an important role in renal development and left-right axis determination. Defects in inversin result in nephronophthisis type 2, an autosomal recessive cystic kidney disease.

~165/12 kDa observed. Uniprot describes 3 isoforms at ~11, ~100, and ~118 kDa The calculated molecular weights are 11 and 118 kDa, however Inversin has been shown as a ~12 and ~165 kDa band in western blots (Nurnberger, J., et al. (2004). J Am Soc Nephrol. 15:1700–1710.). Uncharacterized bands may appear at ~45, ~53, and ~55 kDa

KLH-conjugated linear peptide corresponding to human Inversin.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.