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MAB4360 Anti-TRA-1-60 Antibody, clone TRA-1-60

100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, IF, IP, ICCMPurifiedMonoclonal Antibody
      Catalogue NumberMAB4360
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-TRA-1-60 Antibody, clone TRA-1-60
      Background InformationHuman embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
      Product Information
      • NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
      PresentationPurified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.
      Quality LevelMQ100
      ApplicationThis Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunofluorescence: A previous lot was used in IF.

      Western Blot: A previous lot of this antibody was used in WB.

      Immunoprecipitation: A previous lot of this antibody was used in IP.

      Immunohistochemistry: A previous lot of this antibody was used in IH.

      Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman embryonal carcinoma cell line 2102Ep
      Cloneclone TRA-1-60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-1-60
      Purification MethodAmmonium Sulfate Precipitation
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight235/410 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-TRA-1-60 Antibody, clone TRA-1-60 (M)SDS



      Anti-TRA-1-60 Antibody, clone TRA-1-60 試験成績書(CoA)

      Anti-TRA-1-60, clone TRA-1-60 2462881
      Anti-TRA-1-60, clone TRA-1-60 - 2140039 2140039
      Anti-TRA-1-60, clone TRA-1-60 - 2366550 2366550
      Anti-TRA-1-60, clone TRA-1-60 - 2430441 2430441
      Anti-TRA-1-60, clone TRA-1-60 - 2016625 2016625
      Anti-TRA-1-60, clone TRA-1-60 - 2271178 2271178
      Anti-TRA-1-60, clone TRA-1-60 - 3257043 3257043
      Anti-TRA-1-60, clone TRA-1-60 - 3436989 3436989
      Anti-TRA-1-60, clone TRA-1-60 - 3548341 3548341
      Anti-TRA-1-60, clone TRA-1-60 - 3705692 3705692


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      Nature  2014

      25132547 25132547
      Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
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      PloS one  9  e97397  2014

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      Nature, (2014)  2014

      24776804 24776804
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      Journal of assisted reproduction and genetics  31  983-91  2014

      Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry24938360 24938360


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