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MAB4360 Anti-TRA-1-60 Antibody, clone TRA-1-60

MAB4360
100 µg  
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      概要

      Replacement Information

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      主要スペック表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FC, IF, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4360
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-TRA-1-60 Antibody, clone TRA-1-60
      Background InformationHuman embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
      References
      Product Information
      FormatPurified
      Control
      • NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
      PresentationPurified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunofluorescence: A previous lot was used in IF.

      Western Blot: A previous lot of this antibody was used in WB.

      Immunoprecipitation: A previous lot of this antibody was used in IP.

      Immunohistochemistry: A previous lot of this antibody was used in IH.

      Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman embryonal carcinoma cell line 2102Ep
      Cloneclone TRA-1-60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-1-60
      Purification MethodAmmonium Sulfate Precipitation
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight235/410 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TRA-1-60 Antibody, clone TRA-1-60 (M)SDS

      タイトル

      英語版製品安全データシート((M)SDS) 

      Anti-TRA-1-60 Antibody, clone TRA-1-60 試験成績書(CoA)

      タイトルロット番号
      Anti-TRA-1-60, clone TRA-1-60 2462881
      Anti-TRA-1-60, clone TRA-1-60 - 2140039 2140039
      Anti-TRA-1-60, clone TRA-1-60 - 2366550 2366550
      Anti-TRA-1-60, clone TRA-1-60 - 2430441 2430441
      Anti-TRA-1-60, clone TRA-1-60 - 2016625 2016625
      Anti-TRA-1-60, clone TRA-1-60 - 2271178 2271178
      Anti-TRA-1-60, clone TRA-1-60 - 3257043 3257043
      Anti-TRA-1-60, clone TRA-1-60 - 3436989 3436989
      Anti-TRA-1-60, clone TRA-1-60 - 3548341 3548341
      Anti-TRA-1-60, clone TRA-1-60 - 3705692 3705692

      参考資料

      参考資料の概要アプリケーション動物種Pub Med ID
      A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.
      Chou, BK; Gu, H; Gao, Y; Dowey, SN; Wang, Y; Shi, J; Li, Y; Ye, Z; Cheng, T; Cheng, L
      Stem cells translational medicine  4  320-32  2015

      概要を表示する
      25742692 25742692
      Derivation of induced pluripotent stem cells from orangutan skin fibroblasts.
      Ramaswamy, K; Yik, WY; Wang, XM; Oliphant, EN; Lu, W; Shibata, D; Ryder, OA; Hacia, JG
      BMC research notes  8  577  2015

      概要を表示する
      26475477 26475477
      A method for human teratogen detection by geometrically confined cell differentiation and migration.
      Xing, J; Toh, YC; Xu, S; Yu, H
      Scientific reports  5  10038  2015

      概要を表示する
      25966467 25966467
      An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.
      Nishishita, N; Muramatsu, M; Kawamata, S
      American journal of stem cells  4  38-49  2015

      概要を表示する
      25973330 25973330
      Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
      Cerbini, T; Funahashi, R; Luo, Y; Liu, C; Park, K; Rao, M; Malik, N; Zou, J
      PloS one  10  e0116032  2015

      概要を表示する
      25587899 25587899
      Impaired respiratory function in MELAS-induced pluripotent stem cells with high heteroplasmy levels.
      Kodaira, M; Hatakeyama, H; Yuasa, S; Seki, T; Egashira, T; Tohyama, S; Kuroda, Y; Tanaka, A; Okata, S; Hashimoto, H; Kusumoto, D; Kunitomi, A; Takei, M; Kashimura, S; Suzuki, T; Yozu, G; Shimojima, M; Motoda, C; Hayashiji, N; Saito, Y; Goto, Y; Fukuda, K
      FEBS open bio  5  219-25  2015

      概要を表示する
      25853038 25853038
      Synaptic dysregulation in a human iPS cell model of mental disorders.
      Wen, Z; Nguyen, HN; Guo, Z; Lalli, MA; Wang, X; Su, Y; Kim, NS; Yoon, KJ; Shin, J; Zhang, C; Makri, G; Nauen, D; Yu, H; Guzman, E; Chiang, CH; Yoritomo, N; Kaibuchi, K; Zou, J; Christian, KM; Cheng, L; Ross, CA; Margolis, RL; Chen, G; Kosik, KS; Song, H; Ming, GL
      Nature  2014

      概要を表示する
      25132547 25132547
      Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
      Kishino, Y; Seki, T; Fujita, J; Yuasa, S; Tohyama, S; Kunitomi, A; Tabei, R; Nakajima, K; Okada, M; Hirano, A; Kanazawa, H; Fukuda, K
      PloS one  9  e97397  2014

      概要を表示する
      24824994 24824994
      Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
      Yamada, Mitsutoshi, et al.
      Nature, (2014)  2014

      概要を表示する
      24776804 24776804
      Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes.
      Park, MH; Park, JE; Kim, MS; Lee, KY; Park, HJ; Yun, JI; Choi, JH; Lee, Es; Lee, ST
      Journal of assisted reproduction and genetics  31  983-91  2014

      概要を表示する
      Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry24938360 24938360

      カタログ

      タイトル
      Human Stem Cell Systems

      技術情報

      タイトル
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells

      データシート

      タイトル
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
      STEMCCA Lentivirus Reprogramming Kits

      Posters

      タイトル
      Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein