Cardiac Stem Cell Maintenance Medium

The isolation and expansion of cardiac stem cells opens new opportunities to the feld of cardiac regenerative medicine. CSCs have recently been isolated from human and murine tissues based upon cell biomarkers (Sca-1, TERT, c-Kit, side population), demonstrating the presence of a non-circulating stem cell niche within the myocardium that is estimated to account for 11-14% of the total cell population1. These cells appear to be bi-potent in their capacity to form cardiomyocytes and vascular endothelial cells. Moreover, preliminary engraftment studies suggest that these cells are ideal candidates for future research on cardiac regeneration. However, the rarity of CSCs coupled with the complex isolation procedure is a signifcant challenge for the advancement of the field. o overcome this obstacle, we have developed an easy-to-use cell isolation kit capable of obtaining a high yield, pure population of CSCs. This advancement allows investigators to obtain a signifcantly greater number of CSCs for their studies without the need for time consuming, complex protocols and expensive cell sorting equipment.