Human iPS Reprogramming Boost Supplement II

Research Category
Stem Cell Research

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

EMD Millipore’s Human iPS Reprogramming Boost Supplement II contains three proprietary small molecules (TGF-β RI Kinase Inhibitor IV, Sodium Butyrate and PS48) in amounts sufficient to supplement 350 mL of human ES cell maintenance medium. When used in conjunction with the Human STEMCCA lentivirus reprogramming kits (SCR544, SCR545, and SCR548), the Human iPS Reprogramming Boost Supplement II enhances the efficiency of human iPS colony formation by 10 – 15 fold and shortens the timescale to establish fully reprogrammed colonies by 50%. The colonies formed possess the distinctive flat 2D morphology that are reminiscent of normal human ES cell colonies and can be easily passaged. Fully reprogrammed human iPS colonies that are SSEA-4-positive, TRA-1-60-positive and Hoechst-Dim are readily established in approximately 20 – 25 days.

1. TGF-β RI Kinase Inhibitor IV Supplement (1000X): (Part No. CS210445) One (1) vial containing 400 μL of the inhibitor in high quality DMSO.

2.Sodium Butyrate Supplement (1000X): (Part No. CS210446) One (1) vial containing 400 µL of the inhibitor in water.

3. PS48 Supplement (1000X): (Part No. CS210447) One (1) vial containing 400 L of the inhibitor in high quality DMSO.

Components of the Human iPS Reprogramming Boost Supplement II are stable for at least 6 months from date of receipt when stored at -20°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C. Upon addition of the components of the Mouse iPS Reprogramming Boost Supplement II to the media, filter the supplemented media with a 0.22 µM filtration unit. Supplemented media is good for up to two weeks when stored at 2-8°C.