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Merck

QR0200

定量RT-PCR ReadyMix™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

別名:

1-step RT-qPCR mix, Quantitative real-time PCR master mix

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この商品について

NACRES:
NA.55
UNSPSC Code:
41106300

主要文書

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製品名

定量RT-PCR ReadyMix™, One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

usage

sufficient for 250 reactions

feature

dNTPs included
hotstart

technique(s)

RT-qPCR: suitable

color

colorless

input

purified RNA

compatibility

ABI 5700
 ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT
ABI 7900 HT Fast
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
 Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
 Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex2 s
 Eppendorf® Mastercycler ep realplex
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
 Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

Quality Level

300

関連するカテゴリー

Application

Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced risk of contamination from multiple pipetting steps
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
  • Broad instrument compatibility
  • Includes a separate ROX reference dye vial for reaction normalization

General description

Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Legal Information

This product is for research use only.
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

キットの構成要素のみ

製品番号
詳細
  • Probe Based qRT-PCR ReadyMix 2 X
  • Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

キットの構成要素は別途購入可能です。

製品番号
詳細
SDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vialSDS
  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mMSDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 XSDS

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

保管分類

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
SLCH0651
SLCG8790
SLCG9928
SLCG4184
SLCG4181

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Validation of Quantitative PCR Assays
Lovatt, A., et al.
BioPharm., 22-32 (2002)
A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity
Ji D, et al.
Oncology Reports, 18(6), 1499-1505 (2007)
An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference
Wang, et al.
JALA: Journal of the Association for Laboratory Automation, 11, 314-318 (2006)
S A Bustin
Journal of molecular endocrinology, 29(1), 23-39 (2002-08-30)
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, molecular medicine and biotechnology. Assays are easy to perform, capable of high throughput, and can
T B Morrison et al.
BioTechniques, 24(6), 954-958 (1998-06-19)
Continuous fluorescence observation of amplifying DNA allows rapid and accurate quantification of initial transcript copy number. A simple and generic method for monitoring product synthesis with the double-stranded DNA dye, SYBR Green I provides initial template copy number estimation limited

資料

Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

すべて表示

プロトコル

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

関連コンテンツ

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

GenElute™-E Kits for RNA and DNA Purification

GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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