agency
EP 5.6, JP 4.05, USP 29
form
granular
composition
Dextrose, 4%
manufacturer/tradename
GranuCult® prime
packaging
pack of 500 g and 5 kg
technique(s)
microbiological culture: suitable
pH
5.6 (25 °C, 65 g/L in H2O, after autoclaving)
solubility
65 g/L
bulk density
680 kg/m3
application(s)
bioburden testing
industrial qc
microbiology
pharmaceutical
storage temp.
15-25°C
suitability
molds, yeasts
Quality Level
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General description
Sabouraud 4% Dextrose Agar is a complex, granulated, dehydrated culture medium for cultivation and isolation of yeasts and molds. It also serves as the absence test for Candida albicans. The high concentration of dextrose and the low pH promotes the growth, the formation of spores (Conidia and Sporangia), and the formation of pigments of yeasts and molds. Additionally, it inhibits the growth of bacteria.
Application
Sabouraud 4% Dextrose Agar is designed for the determination of the total count of yeasts and molds in non-sterile products.
Analysis Note
Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Danielle Aparecida Duarte Nunes et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 55(6), 650-660 (2020-02-19)
Bioremediation is one of the existing techniques applied for treating oil-contaminated soil, which can be improved by the incorporation of low-cost nutritional materials. This study aimed to assess the addition of two low-cost plant residues, sugarcane bagasse (SCB) and leaf
資料
Learn more about our culture media portfolio for microbial examination of non-sterile products, fully compliant with the harmonized Pharmacopoeia.
関連コンテンツ
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 1.05438.0500 | 04022536064895 |
| 1.05438.5000 | 04022536064925 |
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