Application
Ethidium bromide has been used to stain polyacrylamide gels for small-scale transcription reactions. It has also been used for the quantification of DNA.
Biochem/physiol Actions
Ethidium bromide is a red fluorescent dye that reacts with the DNA of membrane-damaged cells by intercalating. It is used for visualizing DNA in gels. Ethidium bromide is carcinogenic with moderate toxicity.
Features and Benefits
Robust and easy to use
Analysis Note
Identity (UV/VIS-Spectrum): passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test
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Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Muta. 2
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
保管分類
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
適用法令
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