Hck Protein, activated

34.1kDa

FUNCTION: SwissProt: P08631 # May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
SIZE: 526 amino acids; 59600 Da
SUBUNIT: May bind to HIV-1 Nef and VIF through its SH3 domain. This interaction would stimulates its tyrosine-kinase activity.
SUBCELLULAR LOCATION: Isoform p59-HCK: Membrane; Lipid-anchor. & Isoform p60-HCK: Membrane; Lipid-anchor. Cytoplasm.
TISSUE SPECIFICITY: Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
DOMAIN: SwissProt: P08631 The SH3 domain mediates binding to HIV-1 Nef.
PTM: Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3 (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.

Auto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-843M when ordering the 250 µg size.

Also available in 10 μg size (cat#14-843) and in bulk (cat# 14-843-K).

50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.

6 months at -70°C

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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