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Merck

189490

3-MA

≥95% (HPLC), powder, autophagy inhibitor, Calbiochem®

別名:

Autophagy Inhibitor, 3-MA, 3-Methyladenine

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この商品について

実験式（ヒル表記法）:
C6H7N5
CAS番号:
5142-23-4
分子量:
149.15
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD00010531
Assay:
≥95% (HPLC)
Form:
powder
Quality level:
100
Storage condition:
OK to freeze, protect from light

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製品名

Autophagy Inhibitor, 3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.

SMILES string

N1(C2=NC=NC2=C(N=C1)N)C

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

InChI key

FSASIHFSFGAIJM-UHFFFAOYSA-N

assay

≥95% (HPLC)

form

powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

white to faint yellow

solubility

DMSO: 10 mg/mL, water: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

関連するカテゴリー

General description

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204 ) and Wortmannin (Cat. No. 681675 )

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.

Other Notes

Canu, N., et al. 2005. J. Neurochem.92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

189490-100MG: + 189490-50MG: + 189490-MG:

jan

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Cong Chen et al.
Cell death & disease, 13(2), 150-150 (2022-02-16)
Ferroptosis, which is characterized by intracellular iron accumulation and lipid peroxidation, is a newly described form of regulated cell death that may play a key role in tumour suppression. In the present study, we investigated the expression profiles and biological
Jinghui Zhai et al.
Journal of biochemical and molecular toxicology, 35(11), e22896-e22896 (2021-08-24)
The NOD-like receptor family pyrin domain-containing (NLRP3) inflammasomes is centrally implicated in cisplatin (CP)-induced kidney injury. Autophagy is critical for inhibiting production of NLRP3 protein that effectively reduces the inflammatory response. Ginsenoside Rg3 (SY), an active component extracted from ginseng
Wei Huang et al.
Frontiers in neuroscience, 15, 745815-745815 (2021-12-07)
Parkinson's disease is a neurodegenerative disorder with an inflammatory response as the core pathogenic mechanism. Previous human genetics findings support the view that the loss of TREM2 function will aggravate neurodegeneration, and TREM2 is one of the most highly expressed
Abdelhabib Semlali et al.
Frontiers in oncology, 12, 873447-873447 (2022-10-04)
Treatment of oral cancer is based exclusively on surgery combined with or without chemotherapy. However, it has several side effects. Targeting a new, more effective therapy has become an urgent matter. The purpose of this study was to evaluate the
Nienke Visser et al.
International journal of molecular sciences, 22(5) (2021-03-04)
Elevated activation of the autophagy pathway is currently thought to be one of the survival mechanisms allowing therapy-resistant cancer cells to escape elimination, including for cytarabine (AraC)-resistant acute myeloid leukemia (AML) patients. Consequently, the use of autophagy inhibitors such as
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関連コンテンツ

LentiBrite™ Lentiviral Biosensors for Fluorescent Cellular Imaging: Analysis of Autophagosome Formation

Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.

CARTA DE USO GENERAL 2016

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PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

グローバルトレードアイテム番号

カタログ番号GTIN
189490-100MGCN04055977221831
189490-50MGCN07790788048235

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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