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Merck

20-188

RIPA Lysis Buffer, 10X

100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.

別名:

lysis buffer

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この商品について

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053

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form

liquid

packaging

pkg of 100 mL

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

shipped in

ambient

General description

Radioimmunoprecipitation assay (RIPA) Buffer aids in cell lysis and solubilization of proteins from both adherent and suspension-cultured mammalian cells. In addition, this buffer helps in relevant protein-protein interactions studies by reducing non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur.

Application

RIPA Lysis Buffer, 10X has been used for cell lysis during the sample preparation for immunoprecipitation and western blot.

Physical form

0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.

Preparation Note

6 months at room temperature

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

保管分類

10 - Combustible liquids

wgk

WGK 3

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

20-188:

jan

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Ya-Hui Chen et al.
Cancer management and research, 12, 4645-4665 (2020-07-02)
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with an overall 5-year survival rate of 9.3%, and this malignancy is expected to become the second leading cause of cancer-related death by 2030. Gemcitabine resistance develops within weeks of PDAC
Lena Gamboa et al.
ACS chemical biology, 15(2), 533-542 (2020-01-07)
CRISPR-associated proteins (Cas) are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially defined, noninvasive modalities limits their use as biological tools. Here, we integrate thermal gene switches with dCas9 complexes to confer remote control
Suji Han et al.
Cancers, 11(12) (2019-11-28)
Glioblastoma is a highly aggressive and lethal brain tumor, with limited treatment options. Abnormal activation of the neddylation pathway is observed in glioblastoma, and the NEDD8-activating enzyme (NAE) inhibitor, MLN4924, was previously shown to be effective in glioblastoma cell line
Carla Maiara Lopes Cardozo et al.
Molecules (Basel, Switzerland), 25(11) (2020-06-14)
There are still controversies regarding the correlation between the beneficial effects for health and the administration of isolated compounds or crude extracts in therapeutic applications. Campomanesia xanthocarpa, found in the Brazilian Cerrado, demonstrated beneficial effects in metabolic disorders associated with
Ayodele Akintayo et al.
The Journal of biological chemistry, 295(41), 14053-14064 (2020-08-09)
The membrane-bound, long form of MGAT4D, termed MGAT4D-L, inhibits MGAT1 activity in transfected cells and reduces the generation of complex N-glycans. MGAT1 is the GlcNAc-transferase that initiates complex and hybrid N-glycan synthesis. We show here that Drosophila MGAT1 was also
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グローバルトレードアイテム番号

カタログ番号GTIN
20-18804053252677526

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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