BCIP/NBT

BCIP/NBT provided as a stable single component, ready-to-use substrate, sterile-filtered through a 0.22 µm filter. Specifically designed for immunoblotting techniques.BCIP/NBT is the substrate of choice for alkaline phosphatase-based immunoblotting procedures. Hydrolysis of the phosphate ester of BCIP by alkaline phosphatase yields powerful reducing compounds that react rapidly with NBT and convert it to the insoluble NBT-formazan. This reaction product is readily visible on the blotting membrane as dark purple deposits that do not fade upon drying.

Immunoblotting