264150
Caspase-3 Substrate IV, Fluorogenic
Fluorogenic caspase-3 substrate.
別名:
Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
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この商品について
実験式（ヒル表記法）:
C36H38F3N5O14
CAS番号:
分子量:
821.71
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77
Assay:
≥95% (HPLC)
Form:
solid
Storage condition:
OK to freeze
Quality Segment
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white to off-white
solubility
DMSO: 10 mg/mL
fluorescence
λex ~400 nm, λem ~505 nm
shipped in
ambient
storage temp.
−20°C
SMILES string
FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)OCc3ccccc3)CC(=O)OC)CCC(=O)OC)C(C)C)CC(=O)OC
InChI key
NLDGPABVDARZCY-KIAQLKRMSA-N
General description
Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.
Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Fluorogenic caspase-3 substrate.
Fluorogenic caspase-3 substrate.
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.
Other Notes
Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.
Z-Asp-Glu-Val-Asp-AFC
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable