268298
DAPI, Dihydrochloride
Cell-permeable DNA-binding dye.
別名:
DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
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この商品について
実験式（ヒル表記法）:
C16H15N5 · 2HCl
CAS番号:
分子量:
350.25
UNSPSC Code:
12352200
NACRES:
NA.54
MDL number:
Quality Segment
description
RTECS - NL5995050
assay
≥90% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
yellow
solubility
water: 5 mg/mL, ethanol: soluble, methanol: soluble
fluorescence
λex ~359 nm, λem ~461 nm
shipped in
ambient
storage temp.
−20°C
SMILES string
[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
注意喚起語
Warning
ハザードコード
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
標的臓器
Respiratory system
保管分類
11 - Combustible Solids
一体何が起きているの？
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
268298-MG: + 268298-10MG:
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