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Merck

268298

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

別名:

DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl

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この商品について

実験式（ヒル表記法）:
C16H15N5 · 2HCl
CAS番号:
28718-90-3
分子量:
350.25
UNSPSC Code:
12352200
NACRES:
NA.54
MDL number:
MFCD00012681

主要文書

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SMILES string

[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

description

RTECS - NL5995050

assay

≥90% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

yellow

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

fluorescence

λex ~359 nm, λem ~461 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

268298-MG: + 268298-10MG:

jan

試験成績書（COA）

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Diana Sánchez-Ponce et al.
Cerebral cortex (New York, N.Y. : 1991), 22(7), 1648-1661 (2011-09-24)
The cisternal organelle that resides in the axon initial segment (AIS) of neocortical and hippocampal pyramidal cells is thought to be involved in regulating the Ca(2+) available to maintain AIS scaffolding proteins, thereby preserving normal AIS structure and function. Through
Marta Jiménez-Martínez et al.
Cancers, 11(11) (2019-11-14)
Cell contact inhibition (CCI) is deregulated in cancer. Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. We found that dual-specificity phosphatase 10 (DUSP10) is involved in CRC. DUSP10 overexpression increased the growth of CRC cell lines and
Alexander R H Hobby et al.
American journal of physiology. Heart and circulatory physiology, 317(4), H820-H829 (2019-08-24)
Ischemic heart diseases such as myocardial infarction (MI) are the largest contributors to cardiovascular disease worldwide. The resulting cardiac cell death impairs function of the heart and can lead to heart failure and death. Reperfusion of the ischemic tissue is
Ismael Santa-Maria et al.
Molecular neurodegeneration, 2, 17-17 (2007-09-08)
Alzheimer's disease (AD) is characterized by the presence of two histopathological hallmarks; the senile plaques, or extracellular deposits mainly composed of amyloid-beta peptide (Abeta), and the neurofibrillary tangles, or intraneuronal inclusions composed of hyperphosphorylated tau protein.Since Abeta aggregates are found
Ji Hoon Jeon et al.
Experimental & molecular medicine, 48(12), e277-e277 (2016-12-10)
A small proportion of cancer cells have stem-cell-like properties, are resistant to standard therapy and are associated with a poor prognosis. The metabolism of such drug-resistant cells differs from that of nearby non-resistant cells. In this study, the metabolism of
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LentiBrite™ Lentiviral Biosensors for Fluorescent Cellular Imaging: Analysis of Autophagosome Formation

Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.

グローバルトレードアイテム番号

カタログ番号GTIN
268298-10MGCN04055977197785

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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