Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate

Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.

This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain.

Enzyme Immunoassay (1:1000)

Immunoelectrophoresis (see comments)

Please refer to vial label for lot-specific concentration.

In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.

To achieve maximum enzyme activity, we recommend using the following substrate:

Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl₂) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration.



Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)