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この商品について
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
HP6043, monoclonal
Application:
Citations:
4
製品名
Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate, liquid, clone HP6043, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6043, monoclonal
form
liquid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Quality Level
関連するカテゴリー
Application
Enzyme Immunoassay (1:1000, see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%
There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 10 mg/ml BSA.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
411550-1MG: + 411550B-500UG: + 411550B-0UG: + 411550-500RRUG: + 411550-500UG:
jan
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Farhad Khosravi et al.
Nanotechnology, 27(44), 44LT03-44LT03 (2016-09-30)
We demonstrate the rapid and label-free capture of breast cancer cells spiked in blood using nanotube-antibody micro-arrays. 76-element single wall carbon nanotube arrays were manufactured using photo-lithography, metal deposition, and etching techniques. Anti-epithelial cell adhesion molecule (anti-EpCAM), Anti-human epithelial growth
Farhad Khosravi et al.
Nanotechnology, 27(13), 13LT02-13LT02 (2016-02-24)
We demonstrate the rapid and label-free capture of breast cancer cells spiked in buffy coats using nanotube-antibody micro-arrays. Single wall carbon nanotube arrays were manufactured using photo-lithography, metal deposition, and etching techniques. Anti-epithelial cell adhesion molecule (EpCAM) antibodies were functionalized
Farhad Khosravi et al.
Biosensors, 7(2) (2017-04-20)
This study demonstrates the rapid and label-free detection of Interleukin-6 (IL-6) using carbon nanotube micro-arrays with aptamer as the molecular recognition element. Single wall carbon nanotubes micro-arrays biosensors were manufactured using photo-lithography, metal deposition, and etching techniques. Nanotube biosensors were
Tiina Kelkka et al.
Leukemia, 36(9), 2317-2327 (2022-08-05)
In immune aplastic anemia (IAA), severe pancytopenia results from the immune-mediated destruction of hematopoietic stem cells. Several autoantibodies have been reported, but no clinically applicable autoantibody tests are available for IAA. We screened autoantibodies using a microarray containing >9000 proteins
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 411550-500UGCN | 07790788060275 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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