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484400

Nitrocefin

≥95% (UV), solid, β-lactamase substrate, Calbiochem®

別名:

Nitrocefin, 3-(2,4-Dinitrostyryl)-(6R, 7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic Acid, E-isomer

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この商品について

実験式（ヒル表記法）:
C21H16N4O8S2
CAS番号:
41906-86-9
分子量:
516.50
MDL number:
MFCD12165893
UNSPSC Code:
12352200
NACRES:
NA.21
Assay:
≥95% (UV)
Form:
solid
Storage condition:
OK to freeze, protect from light
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製品名

Nitrocefin, A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.

Quality Segment

200

assay

≥95% (UV)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

orange-yellow

solubility

soluble (Preparing a Nitrocefin (500 µg/ml) Solution
• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.
• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.
• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.
• Nitrocefin, particularly in solution, is very sensitive to light.)

shipped in

ambient

storage temp.

−20°C

SMILES string

[s]1c(ccc1)CC(=O)N[C@H]2[C@H]3SCC(=C(N3C2=O)C(=O)O)\C=C\c4c(cc(cc4)[N+](=O)[O-])[N+](=O)[O-]

InChI

1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1

InChI key

LHNIIDJCEODSHA-OQRUQETBSA-N

General description

A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow (λmax = 390 nm at pH 7.0) to red (λmax = 486 nm at pH 7.0) as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. Also useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. Has been used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.

Packaging

Packaged under inert gas

Preparation Note

Preparing a Nitrocefin (500 µg/ml) Solution

• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.

• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.

• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.

• Nitrocefin, particularly in solution, is very sensitive to light.
Following reconstitution, aliquot and freeze (-20°C). Stock solution may be stored at -20°C for up to 2 weeks.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Guay, R., et al. 1980. IRCS Med. Science 8, 209.
King, A., et al. 1980. Antimicrob. Agents Chemother. 17, 165.
Matthew, M., et al. 1975. J. Gen. Microbiol. 88, 169.
O’Callaghan, C.H., et al. 1972. Antimicrob. Agents Chemother. 1, 283.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Flammable (J)

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

保管分類

11 - Combustible Solids

wgk

WGK 3

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