Phenylmethylsulfonyl Fluoride

Irreversible inhibitor of serine proteases. Its mechanism of action is analogous to that of diisopropylfluorophosphate. PMSF causes sulfonylation of the active-site serine residues. Also reported to inhibit internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor, see AEBSF (Cat. No. 101500).

Cell permeable: no

Primary Target
serine proteases

Product does not compete with ATP.

Reversible: no

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 9 months at 4°C.

t_½ = 1 hour at pH 7.5. Effective concentration: 50 μM.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Seitz, R., et al. 1993. Int. J. Cancer53, 514.
Weaver, V.M., et al. 1993. Biochem. Cell. Biol.71, 488.
Bourgain, R.H., et al. 1992. Adv. Exp. Med. Biol.316, 427.
Chang, C.T., et al. 1992. Biochem. Int.28, 707.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Corrosive (L)