Phosphatase Inhibitor Cocktail III

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

Phosphatase Inhibitor Cocktail Set III has been used:

• as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
• to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
• as a component in cell lysis buffer to extract total proteins for western blotting

Cell permeable: no

Primary Target
Serine/threonine and protein tyrosine phosphatases

Product does not compete with ATP.

Reversible: no

In H₂O.

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

Each vial contains 1 ml of aqueous solution with the following components:

• 50 mM Sodium Fluoride
• 10 mM β-Glycerophosphate (Cat. No. 35675)
• 10 mM Sodium Pyrophosphate Decahydrate
• 1 mM Sodium Orthovanadate

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Toxic (F)