Ru360

A cell-permeable oxygen bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 0.34 nM) and specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (for complete block after incubation with ~10 µM of Ru360 for 30 min. Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction even at micromolar levels.

A cell-permeable oxygen-bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 340 pM). Specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (complete block after incubation with ~10 µM of Ru360 for 30 min). Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction, even at micromolar levels.

Note: 1 set = 10 x 100 µg.

Cell permeable: yes

Primary Target
Ca2+ uptake into mitochondria in vitro

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 184 pM blocking Ca2+ uptake into mitochondria in vitro; and in situ in intact myocytes; Kd = 340 pM for binding mitochondria

Packaged under inert gas

Unstable in solution; reconstitute just prior to use.

Sanchez, J.A., et al. 2001. J. Physiol. 536, 387.
Bassani, R.A., et al. 1998. Cell Calcium23, 433.
Matlib, M.A., et al. 1998. J. Biol. Chem.273, 10223.
Zhou, Z., et al. 1998. J. Physiol.507 (pt 2), 379.
Emerson, J., et al. 1993. J. Am. Chem. Soc.115, 11799.
Ying, W.-L., et al. 1991. Biochemistry30, 4949.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)