565758
β-Secretase Substrate IV, Fluorogenic
≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem®
別名:
β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
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この商品について
実験式（ヒル表記法）:
C91H129N25O25S
CAS番号:
分子量:
2005.22
MDL number:
NACRES:
NA.77
UNSPSC Code:
12352202
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, protect from light
製品名
β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).
Quality Segment
assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
red
solubility
DMSO: 5 mg/mL
fluorescence
λex ~350 nm, λem ~490 nm
shipped in
ambient
storage temp.
−20°C
General description
A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
Biochem/physiol Actions
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
β-secretase substrate
β-secretase substrate
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Other Notes
Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
保管分類
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable