Thrombin, Human Plasma, High Activity

Designed for use in thrombin clotting time tests. Serine protease formed in the plasma during the clotting process from prothrombin, calcium, and thromboplasmin. Acts on the soluble plasma protein fibrinogen to convert it to an insoluble fibrin clot. Prepared from homogenous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Designed for use in thrombin time tests.

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Complete activation from homogeneous prothrombin by SDS-PAGE

Forsberg, G., et al. 1992. J. Protein Chem.11, 201.
Garcia, J.G. 1992. J. Lab. Clin. Med.120, 513.
Scarborough, R.M., et al. 1992. J. Biol. Chem.267, 13146.
Weigel, L.M., et al. 1992. Infect. Immun.60, 1568.

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Toxicity: Harmful (C)