70664
Benzonase® Nuclease
Purity > 99%, Effective viscosity reduction and removal of nucleic acids from protein solutions
別名:
Endonuclease from Serratia marcescens
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この商品について
CAS番号:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Assay:
>99% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
25-29 units/μL
製品名
エンドヌクレアーゼ Serratia marcescens（セラチア菌）由来, Effective viscosity reduction and removal of nucleic acids from protein solutions
biological source
Serratia marcescens
recombinant
expressed in E. coli
assay
>99% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
25-29 units/μL
impurities
<0.25 EU/kU Total endotoxin
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
タンパク質サンプルから核酸を除去する際に使用します。
Biochem/physiol Actions
未変性または熱変性のDNA, RNAを分解します。
Disclaimer
Toxicity: Standard Handling (A)
General description
Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster and PopCulture Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions).Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity> 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains< 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20°C.
Total endotoxin:< 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
The enzyme consists of two subunits of30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions).Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity> 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains< 0.25 EU/1000 units. The product is supplied in 50% glycerol. Store at -20°C.
Total endotoxin:< 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
Effective viscosity reduction and removal of nucleic acids from protein solutions
Other Notes
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
10 - Combustible liquids
wgk
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
70664-UN: + 70664-10KUN: + 70664-250KUN: + 70664-3: + 70664-24KUN: + 70664-100KUN: + 70664-375KUN:
jan
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Erika C Urdaneta et al.
Methods (San Diego, Calif.), 178, 72-82 (2019-10-07)
Post-transcriptional regulation of gene expression in cells is facilitated by formation of RNA-protein complexes (RNPs). While many methods to study eukaryotic (m)RNPs rely on purification of polyadenylated RNA, other important regulatory RNA classes or bacterial mRNA could not be investigated
Timothy N Audam et al.
American journal of physiology. Heart and circulatory physiology, 319(1), H109-H122 (2020-05-23)
Although cell therapy-mediated cardiac repair offers promise for treatment/management of heart failure, lack of fundamental understanding of how cell therapy works limits its translational potential. In particular, whether reparative cells from failing hearts differ from cells derived from nonfailing hearts
Thach H Chu et al.
PLoS pathogens, 16(2), e1008083-e1008083 (2020-02-25)
Antibody functions such as neutralization require recognition of antigen by the Fab region, while effector functions are additionally mediated by interactions of the Fc region with soluble factors and cellular receptors. The efficacy of individual antibodies varies based on Fab
Kee Wui Huang et al.
Frontiers in cellular neuroscience, 14, 65-65 (2020-04-09)
Viral vectors are essential tools for the study of neural circuits, with glycoprotein-deleted rabies viruses being widely used for monosynaptic retrograde tracing to map connectivity between specific cell types in the nervous system. However, the use of rabies virus is
Tian Han et al.
Journal of virology, 94(6) (2020-01-04)
Late gene expression of betaherpesviruses and gammaherpesviruses is tightly controlled by virus-encoded transactivation factors (vTFs). We recently proved that the 6 vTFs of murine cytomegalovirus (MCMV) form a complex to regulate late gene transcription. pM49, one of the vTFs that
資料
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 70664-3CN | 07790788052751 |
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