70850
FastPlax Titer Kit - Novagen
Rapid, reliable titering of baculovirus stocks
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この商品について
NACRES:
NA.54
UNSPSC Code:
12352200
General description
The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.
Packaging
Plastic ampoule
Preparation Note
Multiple storage requirements
Other Notes
- 2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
- 6 ml- 10% Gelatin (contains 0.02% azide)
- 10 µl- FastPlax Antibody
- 300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
- 300 µl- NBT (83 mg/ml in 70% dimethylformamide)
- 300 µl- X-gal (50 mg/ml in dimethylformamide)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS
注意喚起語
Danger
ハザードコード
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
保管分類
3 - Flammable liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
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pdsc
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prtr
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fsl
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ishl_indicated
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ishl_notified
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