71206
Benzonase® Nuclease HC
Purity > 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions
別名:
Endonuclease from Serratia marcescens
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この商品について
CAS番号:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Assay:
>99% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
≥250 units/μL
製品名
エンドヌクレアーゼ Serratia marcescens（セラチア菌）由来, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions
biological source
Serratia marcescens
Quality Level
recombinant
expressed in E. coli
assay
>99% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
≥250 units/μL
impurities
<0.25 EU/kU Total endotoxin
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
General description
Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >99% by SDS-PAGE.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >99% by SDS-PAGE.
Application
タンパク質サンプルから核酸を除去する際に使用します。
Biochem/physiol Actions
未変性または熱変性のDNA, RNAを分解します。
Other Notes
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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保管分類
10 - Combustible liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
このページは、Benzonase®（ベンゾナーゼ）ヌクレアーゼについてよくある質問9項目とその回答をまとめたものです。
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
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グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| 71206-3CN | 04055977271058 |