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Merck

71842

KOD Hot Start Master Mix

Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., ready-to-use solution, suitable for PCR

別名:

High fidelity master mix, Hot start PCR, Hot start master mix

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この商品について

NACRES:
NA.55
UNSPSC Code:
41105500

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製品名

KOD Hot Start Master Mix, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., ready-to-use solution, suitable for PCR

form

ready-to-use solution

usage

sufficient for 100 reactions
 sufficient for 500 reactions

feature

Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

PCR: suitable (2X PCR Master Mix for high fidelity)
PCR: suitable

input

purified DNA

shipped in

dry ice

storage temp.

−20°C

Quality Level

100

関連するカテゴリー

Application

KOD Hot Start Master Mix has been used:
  • To yield PCR product for T7 endonuclease I (T7EI) assay.
  • For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
  • In the PCR amplification of cDNA.
  • In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.

Disclaimer

Toxicity: Standard Handling (A)

Features and Benefits

  • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
  • Amplifies genomic DNA templates up to 12 kbp
  • Amplifies plasmid and lambda DNA template up to 21 kbp
  • Eliminates mispriming and primer-dimer formation
  • Convenient ambient-temperature setup compatible with automation
  • KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
  • Offers high specificity, fidelity and yield during PCR amplification
  • Optimized for ease of use and data reproducibility
  • Simplifies PCR set-up and saves time
  • High consistency with minimal risk of contamination

*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

General description

KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO4. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.

Legal Information

* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

  • 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix

References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
Simply add KOD HotStart® Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart® DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.

保管分類

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

71842-PP: + 71842-PP-15ML: + 71842-1.25EA: + 71842-EA: + 71842-4: + 71842-3:

jan

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

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Adam S Dingens et al.
Cell host & microbe, 21(6), 777-787 (2017-06-06)
Precisely defining how viral mutations affect HIV's sensitivity to antibodies is vital to develop and evaluate vaccines and antibody immunotherapeutics. Despite great effort, a full map of escape mutants has not been delineated for an anti-HIV antibody. We describe a
Jesse D Bloom
Molecular biology and evolution, 31(8), 1956-1978 (2014-05-27)
All modern approaches to molecular phylogenetics require a quantitative model for how genes evolve. Unfortunately, existing evolutionary models do not realistically represent the site-heterogeneous selection that governs actual sequence change. Attempts to remedy this problem have involved augmenting these models
Yesenia Correa et al.
PloS one, 14(12), e0218837-e0218837 (2019-12-21)
The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of
Michael B Doud et al.
Viruses, 8(6) (2016-06-09)
Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths available to the virus. We and others have combined high-throughput mutagenesis with deep sequencing to estimate the effects of
John A Burns et al.
Nucleic acids research, 38(22), 8178-8187 (2010-08-13)
O(6)-Methylguanine (O(6)-meG), which is produced in DNA following exposure to methylating agents, instructs human RNA polymerase II to mis-insert bases opposite the lesion during transcription. In this study, we examined the effect of O(6)-meG on transcription in human cells and
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資料

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

PCRマスターミックス

PCRマスターミックスは、DNAポリメラーゼ、dNTP、MgCl2、バッファーのような成分によりPCR/RT-PCRの反応を簡略化するもので、市販のものも自身で作製するものもあります。

関連コンテンツ

KOD One™ PCR Master Mix

Efficiently amplify your samples with confidence using KOD One™ PCR Master Mix for ultra-fast PCR with one of the fastest elongation rates available for high fidelity polymerases.

TB506 KOD Hot Start Master Mix

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High fidelity gene amplification
PCR Protocols and Guides - Simplify your gene discovery

We strive to provide up-to-date PCR protocols for your greatest experimental challenges. In this guide, we share our collective expertise on technical applications of PCR to help you achieve high fidelity gene amplification using our optimized protocols for minimal sample processing. This guide includes information on sample preparation, primer design, PCR reagent set up, and thermocycling parameters. We’ve provided detailed guidelines for product usage in technical bulletins available on product-specific pages on our website.

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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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