QCM Endothelial Cell Invasion Assay (24 well, colorimetric)

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Introduction
Endothelial cells (EC) invade through the basement membrane (BM) to form sprouting vessels. The invasion process consists of the secretion of matrix metalloproteases (MMP) to degrade basement membrane, the activation of endothelial cells, and the migration of EC across the basement membrane. The understanding of EC invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.

Cell migration may be evaluated through several different methods, the most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses two-chamber system which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are then stained and quantified.

Millipore’s QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert. The level of coating and the pore size is optimized for endothelial cells so the researcher may utilize this kit to mimic physiological condition. After cell invasion has occurred, the researcher can select a staining method to quantify the number of cells that have invaded through the chamber. Millipore offers a colorimetric staining kit (ECM210) and a fluorometric kit staining reagent (ECM211) for both convenience and efficiency.

Research Category
Apoptosis & Cancer

Cell Structure

Sufficient for 24 assays

Store kit materials at 2° to 8°C for up to their expiration date. Do not freeze.

1. Cell Invasion Plate Assembly: (Part No. CS203020) Two 24-well plates each containing 12 ECMatrix-coated 3 m inserts per plate.

2. Cell Stain Solution: (Part No. 90144)* One bottle.

3. Extraction Buffer: (Part No. 90145) One bottle.

4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.

4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.

5. 96-well Stain Quantitation Plate: (Part No. 2005870) One each.

6. Cotton Swabs: (Part No. 10202) Fifty each.

7. Forceps: (Part No. 10203) One each.

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