QCM走化性細胞遊走アッセイ 96ウェル（8 µm） 蛍光測定

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Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

The Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples.

In the Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids.

The Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

The Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay utilizes an 8 μm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.

In addition, Chemicon also provides QCM<TMSYMBOL></TMSYMBOL> 24-well insert cell migration assay systems, CytoMatrix<TMSYMBOL></TMSYMBOL> Cell Adhesion strips coated with ECM proteins or anti integrin antibodies, and QuantiMatrix<TMSYMBOL></TMSYMBOL> ECM protein ELISA kits.

Application:

The Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay is ideal for the study of chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 8 μm pore size of this assay′s Boyden chambers is appropriate for migration studies of most cell types. Each kit provides sufficient materials for the evaluation of 96 samples.

The Chemicon QCM<TMSYMBOL></TMSYMBOL> 96-well Migration Assay is intended for research use only; not for diagnostic applications.

QCM 8 uM 96ウェル遊走アッセイは、白血球の遊走に適している8 umの孔径を使用しています。

研究のカテゴリ
細胞構造

96ウェル

滅菌96ウェル細胞遊走プレートアセンブリ：（部品番号：90128）96ウェルフィーダートレイ1枚、および96ウェル細胞遊走チャンバープレート1枚。面積＝0.3 cm²、細胞50,000個/ウェル

96ウェル細胞培養トレイ：（部品番号：90129）96ウェルフィーダートレイ1枚。

細胞接着溶液：（部品番号：90131）16 mLボトル 1本。

4倍濃縮細胞溶解緩衝液：（部品番号：90130） 16 mLボトル1本。

CyQuant GR Dye：（部品番号：90132）75 μLバイアル1本

CELL COMB is a trademark of Merck KGaA, Darmstadt, Germany

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

メルクのカタログまたは製品に添付されたメルクのその他の文書に記載されていない場合、メルクの製品は研究用途のみを目的としているため、他のいかなる目的にも使用することはできません。このような目的としては、未承認の商業用途、in vitroの診断用途、ex vivoあるいはin vivoの治療用途、またはヒトあるいは動物へのあらゆる種類の消費あるいは適用などがありますが、これらに限定されません。