OVCAR-5 Human Cancer Cell Line

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Ovarian carcinoma is a prevalent worldwide disease, with over 200,000 new cases diagnosed per year (1). Metastatic gastrointestinal cancer frequently presents as advanced ovarian carcinoma (2). The availability of cellular models that recapitulate the spectrum of forms of ovarian cancer are essential to understanding drug resistance, cellular physiology, and advancing new options for treatment.

The OVCAR-5 cell line was established from ascites fluid from a non-treated patient with an advanced-stage ovarian tumor (3). The OVCAR-5 cell line has recently been classified as originating from the gastrointestinal tract, not of ovarian origin (4). OVCAR-5 cells harbor a homozygous Gly12Val mutation in the KRAS oncogene and are characterized by migration/invasion ability as well as exhibiting tumorigenicity in nude mice (5). OVCAR-5 cells overexpress claudin-4, a marker that plays a role in cancer malignancy (6). OVCAR-5 cells exhibit aggressive growth and invasion and represent a valuable model for metastatic gastrointestinal carcinoma.

Source:
OVCAR-5 was established from ascites fluid from a 67-year-old untreated cancer patient (3).

References:
1. Nat Rev Disease Primers 2016; 2: 16061.
2. J Obstet Gynaecol Can 2003; 25(10): 819-824.
3. Cancer Res 1997; 57: 850-856.
4. Nuc Acids Res 2016;44(17): e137.
5. Gynecol Oncol 2015;138(2): 372-377.
6. Int J Mol Sci 2011; 12: 1334-1358.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.