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Merck

1.50657

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

別名:

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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この商品について

UNSPSC Code:
41115709
NACRES:
SB.52

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製品名

SeQuant® ZIC®-cHILIC 3µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-cHILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

1-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

Quality Level

100

関連するカテゴリー

Analysis Note

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

General description

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 100 mm length x 2.1 mm ID is the best choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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資料

Selectivity Examination of Stationary Phases for HILIC

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

プロトコル

LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC

HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

LC/MS Analysis of Nucleotides SeQuant® ZIC®-cHILIC – Effect of pH

ZIC®-cHILIC is a densely bonded zwitterionic stationary phase with phosphorylcholine functional groups covalently attached to silica.

関連コンテンツ

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

低分子HPLC

低分子とは、通常、分子量900ダルトン未満のイオンおよび化合物を指します。これらの化合物は、さまざまな化学的性質（修飾）を有するシリカ粒子またはモノリス型の固定相を主に用いるHPLC、UHPLCおよびLC-MSを用いて、効率よく分離することが可能です。

User Guide (IFU) -1.50657
SeQuant® ZIC®-cHILIC Analytical PEEK coated Columns - Instructions
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グローバルトレードアイテム番号

カタログ番号GTIN
1.50657.000104027269863005

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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