ABE547
Anti-ALKBH5 Antibody
from rabbit, purified by affinity chromatography
別名:
RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
製品名
Anti-ALKBH5 Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ALKBH5(54890)
Analysis Note
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ALKBH5 in 10 µg of MCF-7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ALKBH5 in 10 µg of MCF-7 cell lysate.
Application
Detect ALKBH5 using this rabbit polyclonal antibody, Anti-ALKBH5 Antibody validated for use in western blotting & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot detected ALKBH5 from 500 µg of MCF-7 cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
RNA demethylase ALKBH5 is also known as Alkylated DNA repair protein alkB homolog 5 and Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5. ALKBH5 is a dioxygenase that demethylates RNA, specifically N(6)-methyladenosine (m6A) RNA, which affects mRNA processing and export and is needed for spermatogenesis. ALKBH5 has the highest expression in lung, testis, pancreas, spleen and ovary, but is widely expressed across other tissues.
~52 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ALKBH5.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ye Yuan et al.
Cell death & disease, 12(1), 60-60 (2021-01-13)
ALKBH5 is the main enzyme for m6A-based demethylation of RNAs and it has been implicated in many biological and pathophysiological processes. Here, we aimed to explore the potential involvement of ALKBH5 in osteosarcoma and decipher the underlying cellular/molecular mechanisms. We
RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent manner.
Shen, et al.
Clinical and Translational Medicine, 13, e1279-e1279 (2023)
Zhaofeng Li et al.
Cell death & disease, 12(6), 578-578 (2021-06-06)
N6-methyladenosine (m6A) modification is widespread in messenger RNAs and increasing evidence suggests the crucial roles of m6A in cell differentiation and tissue development. However, whether m6A modulates the osteogenic differentiation of mesenchymal stem cells (MSCs) has not been fully elucidated.
Lan Hong et al.
Cancer chemotherapy and pharmacology, 92(1), 71-81 (2023-06-05)
Tumor-promotive tumor-associated macrophages (TAMs) and the CXCL16/CXCR6 axis have been reported to be correlated with the limited efficacy of chemotherapy in ovarian cancer (OC). However, the role of TAM-secreted CXCL16 and the mechanism by which it affects the cisplatin (DDP)
Rodrigo A Carrasco et al.
Reproduction (Cambridge, England), 162(2), 171-179 (2021-06-16)
To elucidate the mechanism by which nerve growth factor (NGF) influences the LH secretory pathway in camelids, a series of experiments were done to determine the involvement of the hypothalamus (Experiment 1), the role of GnRH neurons (Experiment 2), and
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| ABE547 | 04053252929212 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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