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Merck

MAB1586

Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL

clone R1JHL, Chemicon®, from mouse

別名:

Anti-GluN1, Anti-NDHMSD, Anti-NDHMSR, Anti-NMD-R1, Anti-NMDA1, Anti-NMDAR1, Anti-NR1

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

主要文書

すべての文書を表示
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製品名

Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL, clone R1JHL, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

R1JHL, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

Not specified

NCBI accession no.

NM_000832.5
NM_007327.2
NM_021569.2

UniProt accession no.

Q05586

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... GRIN1(2902)
mouse ... Grin1(14810)
rat ... Grin1(24408)

Application

Detect NMDAR1 using this Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1:300. It is recommended that the antibody be diluted in TBST (20 mM Tris-HCl, 137 mM NaCl, 1% Tween-20, pH 7.6) containing 5% nonfat milk and 0.05% sodium azide. The diluted antibody can be used at least 5 times without a significant signal decrease. Store at 2-8°C between uses.

Immunoprecipitation: 3 μg per 200 μg of lysate will quantitatively precipitate all the NMDA R1 in 200 μg of rat brain lysate.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

NMDA receptor subunit 1 (all splice variants). No cross-reactivity observed with other NMDA receptor subunits. Recognizes a 103-116 kDa protein in rat brain hippocampal homogenate by immunoblot.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: (all splice variants)
Fusion protein of amino acids 1-564 from rat NR1.

Physical form

Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 μL of PBS, pH 7.4. Contains no preservative.
Format: Purified

Preparation Note

Maintain lyophilized material at -20°C for up to six months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

試験成績書（COA）

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文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Nami Tajima et al.
Nature, 534(7605), 63-68 (2016-05-03)
The physiology of N-methyl-d-aspartate (NMDA) receptors is fundamental to brain development and function. NMDA receptors are ionotropic glutamate receptors that function as heterotetramers composed mainly of GluN1 and GluN2 subunits. Activation of NMDA receptors requires binding of neurotransmitter agonists to
María Rodríguez-Muñoz et al.
Oncotarget, 6(34), 35458-35477 (2015-10-16)
In the brain, the histidine triad nucleotide-binding protein 1 (HINT1) and sigma 1 receptors (σ1Rs) coordinate the activity of certain G-protein coupled receptors (GPCRs) with that of glutamate N-methyl-D-aspartate receptors (NMDARs). To determine the role of HINT1-σ1R in the plasticity
Lenka Mikasova et al.
Brain : a journal of neurology, 135(Pt 5), 1606-1621 (2012-05-01)
Autoimmune synaptic encephalitides are recently described human brain diseases leading to psychiatric and neurological syndromes through inappropriate brain-autoantibody interactions. The most frequent synaptic autoimmune encephalitis is associated with autoantibodies against extracellular domains of the glutamatergic N-methyl-d-aspartate receptor, with patients developing
Meilin Tian et al.
Scientific reports, 6, 34751-34751 (2016-10-08)
Allostery is essential to neuronal receptor function, but its transient nature poses a challenge for characterization. The N-terminal domains (NTDs) distinct from ligand binding domains are a major locus for allosteric regulation of NMDA receptors (NMDARs), where different modulatory binding
Katharine R Smith et al.
The Journal of biological chemistry, 292(49), 20173-20183 (2017-10-20)
Brain ischemia causes oxygen and glucose deprivation (OGD) in neurons, triggering a cascade of events leading to synaptic accumulation of glutamate. Excessive activation of glutamate receptors causes excitotoxicity and delayed cell death in vulnerable neurons. Following global cerebral ischemia, hippocampal
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関連コンテンツ

MOUSE ANTI-NMDAR1
Pathways and Biomarkers of Glutamatergic Synapse Flyer

Glutamate is an excitatory neurotransmitter found in the synaptic vesicles of glutamatergic synapses. The post-synaptic neurons in these synapses contain ionotropic and metabotropic glutamate receptors. Glutamate binds to AMPA (α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid) subtype glutamate receptors, leading to sodium influx into the post-synaptic cell and resulting in neuronal excitability and synaptic transmission. The NMDA (N-methyl-d-aspartate) subtype glutamate receptors, on the other hand, regulate synaptic plasticity, and can influence learning and memory. The metabotropic g-protein coupled mGluRs modulate downstream calcium signaling pathways and indirectly influence the synapse’s excitability. The synaptic architecture includes intracellular scaffolding proteins (PSD-95, GRIP), intercellular cell adhesion molecules (NCAMs, N-Cadherins), and a variety of signaling proteins (CaMKII/PKA, PP1/PP2B). Processes critical for synaptic transmission and plasticity are influenced by these molecules and their interactions. When the function of these molecules is disrupted, it leads to synaptic dysfunction and degeneration, and can contribute to dementia as seen in Alzheimer’s disease.

グローバルトレードアイテム番号

カタログ番号GTIN
MAB158604053252664786

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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