SCC116
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
Mouse
別名:
GT1-7 hypothalamic cell line, GT1-7 neural cell line
製品名
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line, GT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.
biological source
mouse
technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
shipped in
ambient
Quality Level
Analysis Note
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.
Application
Research Category
Endocrine
Neuroscience
Endocrine
Neuroscience
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Biochem/physiol Actions
Neural Lineage Cells
General description
GT1-7 is an immortalized mature mouse hypothalamic GnRH neuronal cell line. Immortalized GnRH neurons were generated by introducing a transgene containing the promotor region of the GnRH gene coupled to the coding region of the SV40 T-antigen oncogene into transgenic mice1. The resulting anterior hypothalamic tumors were removed from one of the mice and the cells dissociated and cloned. GT1-7 is a clonal line of mature differentiated GnRH neurons that exhibit high levels of Gnrh1 mRNA and secrete GnRH in response to depolarization.
Gonadotrophin-releasing hormone (GnRH) is a neuropeptide that is synthesized and released from GnRH neurons within the hypothalamus and is required for normal reproductive development and function. The scarcity and scattered distribution of GnRH neurons in the rostral hypothalamus make the biological study of the cells difficult.
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
W C Wetsel et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(9), 4149-4153 (1992-05-01)
Mammalian reproduction is dependent upon intermittent delivery of luteinizing hormone-releasing hormone (LHRH) to the anterior pituitary. This mode of secretion is required to sensitize maximally the gonadotrophs to LHRH stimulation and to regulate gonadotropin gene expression. While LHRH secretion is
Z Liposits et al.
Endocrinology, 129(3), 1575-1583 (1991-09-01)
An immortalized LHRH cell line has recently been developed by genetically targeting these neurons for tumorigenesis. One of the subclones, the GT1-7 cells, was characterized at both the light and electron microscopic levels to study the cellular and subcellular organization
P L Mellon et al.
Neuron, 5(1), 1-10 (1990-07-01)
By genetically targeting tumorigenesis to specific hypothalamic neurons in transgenic mice using the promoter region of the gonadotropin-releasing hormone (GnRH) gene to express the SV40 T-antigen oncogene, we have produced neuronal tumors and developed clonal, differentiated, neurosecretory cell lines. These
A small-molecule degrader of TET3 as treatment for anorexia nervosa in an animal model.
Lv, et al.
Proceedings of the National Academy of Sciences of the USA, 120, e2300015120-e2300015120 (2023)
D B Whyte et al.
Molecular endocrinology (Baltimore, Md.), 9(4), 467-477 (1995-04-01)
The molecular mechanisms specifying gene expression in individual neurons of the mammalian central nervous system have been difficult to study due to the cellular complexity of the brain and the absence of cultured model systems representing differentiated central nervous system
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| SCC116 | 04054839165849 |
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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