Trizma^® 塩酸塩

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

すべてのバッファーのpHは、温度や濃度に依存します。トリスバッファーの場合、温度が1°C低下するとpHは約0.03上昇し、希釈10倍ごとにpHは0.03～0.05低下します。精密なアプリケーションには、適切に校正した、ガラス／カロメル複合電極のpHメーターを使用してください。

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The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany