DUO82064
Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
別名:
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
product line
Duolink®
technique(s)
proximity ligation assay: suitable
fluorescence
λex 360 nm; λem 460 nm
suitability
suitable for fluorescence-detection automated sequencing, suitable for microtiter plates
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
Use the Multiwell Plates modifications to the Duolink® In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Duolink® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink® In Situ in microtiter plates. See the datasheet for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
保管分類
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
資料
実験のTips & Tricks、FAQ、基本的なトラブルシューティングなどのサポート情報。
Duolink™アッセイプロトコルの準備・セットアップ・実施にあたり考慮すべき事項
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
プロトコル
蛍光顕微鏡とMulticolor試薬を使用して、一つのサンプルでタンパク質、タンパク質修飾、もしくはタンパク質間相互作用を最大4つ同時に検出、視覚化そして定量します。
Duolink® PLA Multicolor Detection Protocol
関連コンテンツ
View an automated protocol for Duolink® assays on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
近接ライゲーションアッセイを用いたタンパク質間相互作用、翻訳後修飾、低発現タンパク質の検出、定量および可視化などの応用例
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）