MAK052
ALT Activity Assay
sufficient for 100 colorimetric or fluorometric tests
別名:
Alanine Aminotransferase Activity Assay Kit, SGPT Activity Assay Kit, Serum Glutamic-Pyruvic Transaminase Activity Assay Kit
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)
General description
Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.
Application
Suitable for the measurement of alanine aminotransferase (ALT) activity in biological samples including tissue, cells and serum.
ALT Activity Assay kit has been used to determine the activity of alanine aminotransferase (ALT) in serum samples.
Biochem/physiol Actions
The ALT Activity Assay Kit provides a simple and direct procedure for measuring ALT activity in a variety of biological samples.
ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 μmole of pyruvate per minute at 37 °C.
ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 μmole of pyruvate per minute at 37 °C.
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
保管分類
10 - Combustible liquids
wgk
WGK 3
適用法令
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pdsc
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prtr
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fsl
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ishl_indicated
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ishl_notified
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キットコンポーネントの情報を参照してください
jan
Melissa F Brereton et al.
Nature communications, 7, 13496-13496 (2016-11-25)
Insulin secretion from pancreatic β-cells is impaired in all forms of diabetes. The resultant hyperglycaemia has deleterious effects on many tissues, including β-cells. Here we show that chronic hyperglycaemia impairs glucose metabolism and alters expression of metabolic genes in pancreatic
Nan Wu et al.
Laboratory investigation; a journal of technical methods and pathology, 97(7), 843-853 (2017-06-06)
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Resolvin D1 and E1 alleviate the progress of hepatitis toward liver cancer in long-term concanavalin A-induced mice through inhibition of NF-KB activity.
Kuang H, et al.
Oncology Reports, 35(1), 307-317 (2016)
Investigation of Therapeutic Effects of $\alpha$-Mangostin on Thioacetamide-Induced Cirrhosis in Rats
Nilbunga S.
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 98(9), S91-S97 (2015)
Brett Palaschak et al.
Molecular therapy. Methods & clinical development, 5, 142-152 (2017-05-10)
Multiple independent adeno-associated virus (AAV) gene therapy clinical trials for hemophilia B, utilizing different AAV serotypes, have reported a vector dose-dependent loss of circulating factor IX (FIX) protein associated with capsid-specific CD8
関連コンテンツ
Instructions
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