P2192
PCRバッファー 10X
Optimized for routine PCR with MgCl2 included
別名:
PCR 反応バッファー, PCR 緩衝液
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この商品について
NACRES:
NA.52
UNSPSC Code:
41106306
Quality Level
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Application
10倍PCRバッファーは、PCRミックスの成分として以下の用途に使用されています：
- ウイルスDNAの増幅
- 注意欠陥多動性障害（ADHD）患者におけるメチレンテトラヒドロ葉酸還元酵素（MTHFR）遺伝子変異を検討するためのゲノムDNA増幅
- Bacillus属菌種由来16S-23S rRNA遺伝子の内部転写スペーサー（ITS）増幅
Features and Benefits
- スタンドアロンのバッファー
- JumpStart™ Taq DNA Polymerase（D9307）、Thermus aquaticus由来のTaq DNA Polymerase（D1806）、及びREDTaq® Genomic DNA Polymerase（D8312）に適合します
Preparation Note
10×緩衝液の組成: 100 mM トリス塩酸、25°CでpH 8.3、500 mM KCl、15 mM MgCl2、0.01%ゼラチン
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
プロトコル
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
関連コンテンツ
Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
Thao N T P, et al.
Open Journal of Genetics, 2014 (2014)
Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.