P2192
PCRバッファー 10X
Optimized for routine PCR with MgCl2 included
別名:
PCR 反応バッファー, PCR 緩衝液
製品名
PCRバッファー 10X, Optimized for routine PCR with MgCl2 included
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Quality Level
関連するカテゴリー
Application
10倍PCRバッファーは、PCRミックスの成分として以下の用途に使用されています：
- ウイルスDNAの増幅
- 注意欠陥多動性障害（ADHD）患者におけるメチレンテトラヒドロ葉酸還元酵素（MTHFR）遺伝子変異を検討するためのゲノムDNA増幅
- Bacillus属菌種由来16S-23S rRNA遺伝子の内部転写スペーサー（ITS）増幅
Features and Benefits
- スタンドアロンのバッファー
- JumpStart™ Taq DNA Polymerase（D9307）、Thermus aquaticus由来のTaq DNA Polymerase（D1806）、及びREDTaq® Genomic DNA Polymerase（D8312）に適合します
Preparation Note
10×緩衝液の組成: 100 mM トリス塩酸、25°CでpH 8.3、500 mM KCl、15 mM MgCl2、0.01%ゼラチン
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
Thao N T P, et al.
Open Journal of Genetics, 2014 (2014)
Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Cem Gokcen et al.
International journal of medical sciences, 8(7), 523-528 (2011-09-08)
The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
プロトコル
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
関連コンテンツ
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