ピルビン酸 オキシダーゼ from Aerococcus sp.

Pyruvate Oxidase from Aerococcus sp. has been used in the amplex Red-based fluorescence assay for pyruvate. It is suitable for use in the preparation of biosensor for determination of thiamine (vitamin B1), and as a component in the constant part of the multi-enzyme biocatalytic cascade for the determination of biomarkers for traumatic brain injury (TBI) and soft tissue injury (STI).

Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO₂. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Streptococcus pneumoniae.

Pyruvate Oxidase oxidises pyruvate to form acetate, hydrogen peroxide and carbon dioxide. Pyruvate oxidase requires flavin adenine dinucleotide (FAD), thiamine pyrophosphate (TPP) and magnesium as cofactors for its catalytic activity. Thiamine activates pyruvate oxidase activity.

Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.

凍結乾燥品(バッファ-塩と糖を含有)

1ユニットは、pH 6.7、37°Cでピルビン酸とリン酸がアセチルリン酸とCO₂へと変換される反応において、1分間で、1.0 μmolのH₂O₂を生成する酵素量です。