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リステリア属とL. monocytogenesの検査


L. monocytogenesとリステリア属検出用の


L. monocytogenesは、既知のリステリア属のうち世界に最も広く分布している食品媒介病原菌の1つです。リステリアは一般に冷蔵温度(2~8℃)で増殖できるため、惣菜やインスタント食品の汚染の原因になることが増えてきています。食品のリスク関連の品質管理において、 L. monocytogenesリステリア属全般に対する検査が必要となります。リステリア、特に L. innocuaの存在が、生産工程での重要な衛生状況の指標となります。

Merckは、リステリア属および L. monocytogenes を増菌、検出および分離するための革新的な製品を幅広く取り揃えています。具体的には、以下の通りです。

  • 顆粒状培地:ISO 11133に規定された業界最高レベルの性能基準を満たした、独自の便利な顆粒状培地食中毒菌検査のルーチン作業をスピードアップおよび簡素化するために、リステリア菌迅速簡易検査用の秤量・γ線照射済みReadybag®培地パウチも現在提供しています。
  • 私たちの最新のイノベーションは、食中毒菌検査ルーチンをスピードアップおよび簡素化する、秤量・γ線照射済みReadybag®培地パウチです。
  • シングルパス® ラテラルフロー検査:増菌ステップ後わずか20分で検出できる、非常に便利な「妊娠検査キット」と同原理の革新的な免疫検査法


Method ISO 11290-1 FDA-BAM Chapter 10 FSIS MLG 8.07
Pre-enrichment Half Fraser Broth, 30±2°C, 24±3 h bLEB base broth, 30°C, 4 h UVM, 30±°2C, 22±2 h
Selective enrichment Fraser Broth, 35-37°C,
48±3 h
bLEB w. selective agents added, 30°C, 20 h (44 h) MOPS-bLEB, 35±2°C, 22±2 h or
Fraser Broth, 35±2°C,
24 h (48 h)
Isolation ALOA agar + additional, 24±3 h /
(48±3 h)
Oxford, Palcam, Chromocult Listeria, 35°C, 24 – 48 h MOX-agar, 35±2°C, 24±2 h

Listeria is a genus of Gram-positive, non-sporeforming, rod-shaped bacteria. Of the known species of the genus Listeria, Listeria monocytogenes deserves particular mention as a human and animal pathogen; L. ivanovii is mainly pathogenic in animals, while the remaining species are considered harmless environmental bacteria. They are characterized by several factors, including their ability to grow at refrigeration temperatures (2–8°C), causing contamination of ready-to eat foods to pose a serious risk.

Consequently, food legislation in many countries specifies strict limits for Listeria spp. or Listeria monocytogenes. In the conduct of risk-related quality controls in food, tests should be run for L. monocytogenes, and for the Listeria Genus in general.

The presence of Listeria – in particular of L. innocua – is an indicator for critical hygienic conditions in the production process. A drastic increase in the incidence of food infection caused by Listeria has resulted in a demand for reliable and rapid methods of detection. Apart from traditional culture methods, immunological techniques are becoming ever more popular with users due to their superior specificity and sensitivity.


Listeria monocytogenes is one of the most widely distributed foodborne pathogens in the world and is responsible for severe infections in immunocompromised persons, pregnant women and neonates. As a result, the mortality rate in infected individuals is high despite the relatively low number of cases around the world.

Listeria infections (listeriosis) can result in anything from mild gastroenteritis to severe cases of sepsis, meningitis, encephalitis or abortion. Due to the ubiquitous distribution of Listeria and their capability to grow at refrigeration temperatures (2–8°C), food products constitute one of the main sources of infection.


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